Open AccessComparison of nonstructural protein-1 antigen detection by rapid and enzyme-linked immunosorbent assay test and its correlation with polymerase chain reaction for early diagnosis of dengue
Author(s) -
Seema Gaikwad,
Sandhya Sawant,
Jayanthi Shastri
Publication year - 2017
Publication title -
journal of laboratory physicians
Language(s) - English
Resource type - Journals
eISSN - 0974-7826
pISSN - 0974-2727
DOI - 10.4103/0974-2727.208265
Subject(s) - dengue fever , polymerase chain reaction , virology , antigen , biology , immunology , gene , biochemistry
Early diagnosis of dengue is important for appropriate clinical management and vector control. Different serological tests based on the principle of immunochromatography and enzyme-linked immunosorbent assay (ELISA) are commonly used for detection of antigen and antibodies of dengue virus. The performance of these tests depends on the sensitivity and specificity. Hence, the study was undertaken to compare nonstructural protein-1 (NS1) antigen detection by rapid and ELISA with real-time polymerase chain reaction (RT-PCR) for diagnosis of dengue.