Open AccessBirth after human chorionic gonadotropin-primed oocyte in vitro maturation and fertilization with testicular sperm in a normo-ovulatory patient
Author(s) -
Claudia González-Ortega,
Raúl E. Piña-Aguilar,
Patricia Cancino-Villareal,
Antonio Martín Gutiérrez-Gutiérrez
Publication year - 2016
Publication title -
journal of human reproductive sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.484
H-Index - 31
eISSN - 0974-1208
pISSN - 1998-4766
DOI - 10.4103/0974-1208.192076
Subject(s) - intracytoplasmic sperm injection , andrology , human chorionic gonadotropin , sperm , oocyte , germinal vesicle , human fertilization , in vitro fertilisation , in vitro maturation , zygote , biology , medicine , embryogenesis , pregnancy , embryo , anatomy , genetics
In this report, we present a case of in vitro maturation (IVM) with surgical retrieved testicular sperm in a normo-ovulatory female. Human chorionic gonadotropin-primed IVM, testicular biopsy for sperm retrieval and intracytoplasmic sperm injection with fresh sperm were performed. Fourteen cumulus-oocyte complexes were obtained in germinal vesicle or metaphase I stage, eight oocytes reached metaphase II, seven presumptive zygotes were obtained, and three cleavage stages embryos in day 2 were transferred producing a singleton pregnancy. A single healthy newborn was obtained. Our results suggest that IVM may be an alternative for in vitro fertilization in normo-ovulatory women even if surgical retrieval of sperm is needed. Further research is required to depict contributing factors to the success of IVM in indications different from polycystic ovaries syndrome and the role of male gamete.