Open AccessAssociation of large scale 4977-bp "common" deletions in sperm mitochondrial DNA with asthenozoospermia and oligoasthenoteratozoospermia
Author(s) -
Prafulla S Ambulkar,
Ajay R Chuadhari,
Asoke K Pal
Publication year - 2016
Publication title -
journal of human reproductive sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.484
H-Index - 31
eISSN - 0974-1208
pISSN - 1998-4766
DOI - 10.4103/0974-1208.178635
Subject(s) - asthenozoospermia , biology , percoll , sperm , male infertility , andrology , sperm motility , mitochondrial dna , polymerase chain reaction , oligospermia , genetics , semen , microbiology and biotechnology , gene , infertility , medicine , pregnancy , in vitro
To determine the association of large-scale mitochondrial DNA (mtDNA) deletions with abnormal sperm or abnormal flagellar movement of human spermatozoa in asthenozoospermia and oligoasthenoteratozoospermia (OAT) subjects using percoll gradients fractionation and long-range polymerase chain reaction (PCR).