Open AccessDetection of T and B cells specific complement-fixing alloantibodies using flow cytometry: A diagnostic approach for a resource limited laboratory
Author(s) -
Dharmendra Jain,
Pranav Dorwal,
Amit Pande,
Neetu Tyagi,
Simmi Mehra,
Vimarsh Raina
Publication year - 2017
Publication title -
asian journal of transfusion science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.262
H-Index - 22
eISSN - 1998-3565
pISSN - 0973-6247
DOI - 10.4103/0973-6247.214355
Subject(s) - medicine , flow cytometry , human leukocyte antigen , antibody , immunology , kidney transplantation , transplantation , isoantibodies , b cell , antigen , peritubular capillaries
Various methods have been reported for the detection of antibodies in recipient sera, which can be human leukocyte antigens (HLAs) or non-HLA specific, complement- or noncomplement fixing, as well as donor T (HLA-Class-I) and/or B cell (HLA-Class-I and II) specific. These alloantibodies play a pivotal role in antibody-mediated renal transplantation rejection. Deposition of C4d in peritubular capillaries of a kidney biopsy is a marker of antibody-mediated rejection. The C4d flow-panel reactive antibodies (PRAs) are a screening method for HLA-specific and complement fixing antibodies. However, the method is limited by the lack of donor specificity.