Open AccessRapid determination of paeoniflorin fromPaeonia sinjiang K. Y. Pan.by rapid resolution liquid chromatography
Author(s) -
Xiaoxin Zhang,
Xiaoming Chen,
Liang Ge,
Hao Gong,
Shuge Tian,
AN Dong-qing
Publication year - 2010
Publication title -
pharmacognosy magazine
Language(s) - English
Resource type - Journals
eISSN - 0976-4062
pISSN - 0973-1296
DOI - 10.4103/0973-1296.62893
Subject(s) - paeoniflorin , monobasic acid , chromatography , chemistry , resolution (logic) , potassium , potassium phosphate , high performance liquid chromatography , relative standard deviation , methanol , paeonia lactiflora , analytical chemistry (journal) , detection limit , medicine , pathology , artificial intelligence , computer science , polymer chemistry , alternative medicine , organic chemistry
A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C(18) 4.6 mm x 50 mm, 1.8 mum) column with mobile phase complosed of methanol and 0.05 mol/l potassium phosphate monobasic. Flow rate was at 1.0 ml/min. Retention time was at about 1.16 min. Recovery rate was 99.8% and relative standard deviation of three replicate samples tested was 1.8%. RRLC method has been applied to both in vitro studies of paeoniflorin formulation and drug estimation in biological samples.