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To simulate the ischemia-reperfusion injury in vivo, hypoxia/reoxygenation injury model was established in vitro and primary cultured neonatal rat cardiomyocytes were underwent hypoxia with hydrosulfite (Na2S2O4) for 1 h followed by 1 h reoxygenation.

pharmacognosy magazine

Comparison of the protective effects of ferulic acid and its drug - containing plasma on primary cultured neonatal rat cardiomyocytes with hypoxia/reoxygenation injury