Open AccessModified low cost SNP genotyping technique using cycle threshold (Ct) & melting temperature (Tm) values in allele specific real-time PCR
Author(s) -
D Benet Bosco Dhas,
A Hiasindh Ashmi,
Binu Bhat,
Parija Sc,
N Banupriya
Publication year - 2015
Publication title -
indian journal of medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.578
H-Index - 87
ISSN - 0971-5916
DOI - 10.4103/0971-5916.171282
Subject(s) - genotyping , zygosity , allele , melting curve analysis , agarose gel electrophoresis , single nucleotide polymorphism , biology , genotype , polymerase chain reaction , allele frequency , microbiology and biotechnology , genetics , snp genotyping , variants of pcr , dna , gene
Genotyping has now become one of the major diagnostic means for almost all diseases. Among the advanced techniques that are used to study single nucleotide polymorphisms (SNPs), only a few are applicable for routine disease diagnosis. Their applicability mainly depends on three factors: cost, time, and accuracy. The primary objective of this study was to propose allele-specific real-time PCR as a rapid, low cost and simple genotyping method for routine diagnostics.