Open AccessIn vitro Flow Perfusion Maintaining Long-term Viability of the Rat Groin Fat Flap
Author(s) -
Yang An,
Fangfei Nie,
Zelian Qin,
Xue Han,
Lu-Jia Chen,
Li Bi,
Li Dong
Publication year - 2018
Publication title -
chinese medical journal/chinese medical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 63
eISSN - 2542-5641
pISSN - 0366-6999
DOI - 10.4103/0366-6999.222334
Subject(s) - perfusion , bioreactor , calcein , adipose tissue , viability assay , chemistry , biology , anatomy , in vitro , medicine , biochemistry , membrane , organic chemistry
Large-scale muscle tissue engineering remains a major challenge. An axial vascular pedicle and perfusion bioreactor are necessary for the development and maintenance of large-scale engineered muscle to ensure circulation within the construct. We aimed to develop a novel experimental model of a large-scale engineered muscle flap from an existing rat groin fat flap.