Open AccessDiagnosis of Aspergillus fumigatus endophthalmitis from formalin fixed paraffin-embedded tissue by polymerase chain reaction-based restriction fragment length polymorphism
Author(s) -
Jyotirmay Biwas,
R Bagyalakshmi,
Lily Therese
Publication year - 2008
Publication title -
indian journal of ophthalmology/indian journal of ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.542
H-Index - 51
eISSN - 1998-3689
pISSN - 0301-4738
DOI - 10.4103/0301-4738.37600
Subject(s) - aspergillus fumigatus , restriction fragment length polymorphism , polymerase chain reaction , aspergillus , medicine , endophthalmitis , fungus , microbiology and biotechnology , amplified fragment length polymorphism , fungi imperfecti , polymorphism (computer science) , biology , genetics , genotype , gene , ophthalmology , botany , population , genetic diversity , environmental health
New molecular biological technique of Polymerase Chain Reaction (PCR) based Restriction Fragment Length Polymorphism (RFLP) can identify the species from paraffin-embedded tissue section. We demonstrated Aspergillus fumigatus fungus by PCR-based RFLP technique from paraffin section of an eyeball of an eight-month-old child removed for endogenous endophthalmitis.