Open AccessBAX/BCL-2 mRNA and protein expression in human breast MCF-7 cells exposed to drug vehicles-methanol and dimethyl sulfoxide (DMSO) for 24 hrs
Author(s) -
Gbenga Anthony Adefolaju,
Kathrine E. Theron,
Margot J. Hosie
Publication year - 2015
Publication title -
nigerian medical journal/nigerian medical journal
Language(s) - English
Resource type - Journals
eISSN - 2229-774X
pISSN - 0300-1652
DOI - 10.4103/0300-1652.160349
Subject(s) - dimethyl sulfoxide , mcf 7 , in vitro , apoptosis , toxicity , microbiology and biotechnology , cytotoxic t cell , chemistry , gene expression , methanol , messenger rna , cytotoxicity , viability assay , cancer cell , biochemistry , biology , gene , human breast , cancer , organic chemistry , genetics
Methanol and DMSO are commonly used as carrier solvents for lipophilic chemicals in in-vitro experiments. However, very little information is available regarding the effects of these solvents on the expression of pro and anti-apoptotic genes and proteins.