Open AccessSuccessful pregnancy outcome after in vitro fertilisation following Pre-implantation Genetic Diagnosis/Polymerase Chain Reaction screening for single gene disorder (sickle cell anaemia) before embryo transfer: The clinical experience of an in vitro fertilisation clinic in Nigeria
Author(s) -
Chizara C. Okeke,
K Ailoje-Ibru,
Kemi Olukoya,
Rose Ogbeche,
Abiola Adewusi,
Ebele C. Iloabachie,
Oladapo A. Ashiru
Publication year - 2014
Publication title -
nigerian medical journal/nigerian medical journal
Language(s) - English
Resource type - Journals
eISSN - 2229-774X
pISSN - 0300-1652
DOI - 10.4103/0300-1652.128181
Subject(s) - polymerase chain reaction , pregnancy , genotype , in vitro fertilisation , offspring , biology , embryo , sickle cell trait , embryo transfer , genetic testing , preimplantation genetic diagnosis , andrology , genetics , prenatal diagnosis , medicine , disease , fetus , gene
A couple, both carriers of the sickle cell anaemia trait (Genotype HbAS) with an offspring already affected with the genetic disease underwent a Pre-implantation Genetic Diagnosis/Polymerase Chain Reaction screening of biopsied blastomeres. DNA analysis of single blastomeres was carried out to find out indicated a viable intra-uterine pregnancy with embryos which carried the sickle cell mutation, which resulted in a livebirth (HbAS). PGD/PCR in combination with IVF appears to be the most suitable treatment plan for patients who are at a higher risk of reproducing offspring affected with inheritable genetic diseases.