Open AccessIsolation and characterization of impurities present in 8-chlorotheophylline
Author(s) -
Sonal Desai,
Samir Patel,
Satish Y. Gabhe
Publication year - 2011
Publication title -
indian journal of pharmaceutical sciences/indian journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.245
H-Index - 57
eISSN - 1998-3743
pISSN - 0250-474X
DOI - 10.4103/0250-474x.89762
Subject(s) - impurity , purine , characterization (materials science) , chemistry , chromatography , mass spectrometry , phase (matter) , analytical chemistry (journal) , materials science , organic chemistry , enzyme , nanotechnology
A simple isocratic reversed phase high performance liquid chromatography was used to separate three impurities present in the sample of 8-chlorotheophylline. LC-MS was used for the characterization of impurities. Based on mass spectral data, the structures of these impurities were characterized as 3,7-dihydro-1,3-dimethyl-1H-purine-2,6-dione (impurity I), 3,7-dihydro-1,3,7-trimethyl-1H-purine-2,6-dione (impurity II) and isomer of 8-chloro-1,3-dimethyl-2,6(3H,1H)-purinedione (impurity III).