Open AccessTransactivating effect of complete S protein of hepatitis B virus and cloning of genes transactivated by complete S protein using suppression subtractive hybridization technique
Author(s) -
Guiqin Bai,
Yan Liu,
Jun Cheng,
Shulin Zhang,
Yafei Yue,
Yanping Huang,
Liying Zhang
Publication year - 2005
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v11.i25.3893
Subject(s) - suppression subtractive hybridization , microbiology and biotechnology , complementary dna , biology , cdna library , restriction enzyme , rapid amplification of cdna ends , gene , hepatitis b virus , virology , virus , molecular cloning , genetics
To investigate the transactivating effect of complete S protein of hepatitis B virus (HBV) and to construct a subtractive cDNA library of genes transactivated by complete S protein of HBV by suppression subtractive hybridization (SSH) technique and to clone genes associated with its transactivation activity, and to pave the way for elucidating the pathogenesis of hepatitis B virus infection.