Open AccesssiRNA-mediated inhibition of HBV replication and expression
Author(s) -
Xiaonan Zhang,
Wei Xiong,
Jia-Dong Wang,
Yunwen Hu,
Xiang Li,
Zhenghong Yuan
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i20.2967
Subject(s) - rna interference , small interfering rna , rna silencing , biology , microbiology and biotechnology , rna , small hairpin rna , transfection , gene expression , virology , expression vector , dna directed rna interference , cell culture , gene , recombinant dna , genetics
RNA interference (RNAi) is a newly discovered phenomenon provoked by dsRNA. The dsRNA is initially cleaved by Dicer into 21-23 nt small interfering RNA (siRNA) and can then specifically target homologous mRNA for degradation by cellular ribonucleases. RNAi has been successfully utilized to down-regulate the endogenous gene expression or suppress the replication of various pathogens in mammalian cells. In this study, we investigated whether vector-based siRNA promoted by U6 (pSilencer1.0-U6) could efficiently inhibit HBV replication in cell culture.