Open AccessIsolation and characterization of microsatellite loci from Arthropodium cirratum (Asparagaceae)
Author(s) -
Bulgarella Mariana,
Biggs Patrick J.,
de Lange Peter J.,
Shepherd Lara D.
Publication year - 2017
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1700041
Subject(s) - asparagaceae , biology , microsatellite , locus (genetics) , allele , genetics , population genetics , evolutionary biology , population , gene , botany , demography , sociology
Premise of the study: Microsatellite markers were developed for Arthropodium cirratum (Asparagaceae) to study population genetic structure and translocation of this species. These markers were tested for cross‐amplification in two other Arthropodium species. Methods and Results: Sixteen microsatellite markers were developed from a genomic library and tested in three populations of A. cirratum . The loci exhibited one to five alleles per locus, with private alleles present in each of the populations. Cross‐amplification tests in the two other New Zealand Arthropodium species revealed that many of the loci amplify and demonstrate polymorphism in A. bifurcatum . Conclusions: These markers will be useful for determining genetic structure in A. cirratum and for determining the origins of translocated populations of this species.