Open AccessPrimers for Castilleja and their utility across Orobanchaceae: II. Single‐copy nuclear loci 1
Author(s) -
Latvis Maribeth,
Jacobs Sarah J.,
Mortimer Sebastian M. E.,
Richards Melissa,
Blischak Paul D.,
Mathews Sarah,
Tank David C.
Publication year - 2017
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1700038
Subject(s) - biology , orobanchaceae , clade , evolutionary biology , roseobacter , genetics , primer (cosmetics) , phylogenetic tree , gene , botany , physics , thermodynamics
Premise of the study: We developed primers targeting nuclear loci in Castilleja with the goal of reconstructing the evolutionary history of this challenging clade. These primers were tested across other major clades in Orobanchaceae to assess their broader utility. Methods and Results: We assembled low‐coverage genomes for three taxa in Castilleja and developed primer combinations for the single‐copy conserved ortholog set (COSII) and the pentatricopeptide repeat (PPR) gene family. These primer combinations were designed to take advantage of the Fluidigm microfluidic PCR platform and are well suited for high‐throughput sequencing applications. Eighty‐seven primers were designed for Castilleja , and 27 were found to have broader utility in Orobanchaceae. Conclusions: These results demonstrate the utility of these primers, not only across Castilleja , but for other lineages within Orobanchaceae as well. This expanded molecular toolkit will be an asset to future phylogenetic studies in Castilleja and throughout Orobanchaceae.