Open AccessDevelopment of microsatellite markers for Eurya acuminatissima (Theaceae)
Author(s) -
Shi Xianggang,
Wu Haidan,
Xie Hongxian,
Zheng Yi,
Huang Yelin,
Yu Shixiao
Publication year - 2017
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1700037
Subject(s) - biology , microsatellite , locus (genetics) , allele , genetic diversity , evolutionary biology , genetics , gene , population , demography , sociology
Premise of the study: Sixteen microsatellite markers were developed to study the fine‐scale spatial genetic structure of Eurya acuminatissima , a dioecious tree species of Theaceae endemic to southern China. Methods and Results: A total of 30 primer pairs were initially designed and tested on the basis of the transcriptome data of E. acuminatissima , of which 16 were successfully amplified and showed clear polymorphism. For these microsatellites, one to 17 alleles per locus were identified. The observed and expected heterozygosities ranged from 0 to 1.000 and 0 to 0.903, respectively. Conclusions: The microsatellite markers described here can be used to study genetic diversity and spatial genetic structure of E. acuminatissima . Furthermore, all loci were successfully cross‐amplified in a related species, E. auriformis .