Open AccessMicrosatellites for Phytolacca acinosa (Phytolaccaceae), a traditional medicinal herb 1
Author(s) -
Cheng Yueqin,
Li Pingping,
Yang Yi,
Zhang Jianhang,
Yan Fengming,
Wang Hongwei
Publication year - 2017
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1700028
Subject(s) - biology , loss of heterozygosity , microsatellite , botany , genetic diversity , population , locus (genetics) , herb , medicinal herbs , allele , traditional medicine , gene , genetics , medicine , demography , sociology
Premise of the study: Phytolacca acinosa (Phytolaccaceae) is a traditional Chinese herb with multiple medicinal uses and is an important pigment source. Due to excessive human harvesting, the population numbers and sizes have decreased dramatically. Methods and Results: Using an enriched genomic library, we developed and characterized 15 microsatellite primers for P. acinosa , 13 of which were polymorphic. The number of alleles varied from two to seven. The observed heterozygosity and expected heterozygosity per locus ranged from 0.267 to 1.000 and 0.331 to 0.743, respectively. All of the primers that were developed were also successfully applied in P. americana . Conclusions: These markers should be useful in probing the genetic diversity, genetic structure, and mating systems of P. acinosa , which could provide information about protecting and sustainably harvesting this species.