Open AccessDevelopment and characterization of EST‐SSR markers in Stipa breviflora (Poaceae)
Author(s) -
Ren Jing,
Su ZhiZhen,
Dang ZhenHua,
Ding Yu,
Wang PeiXuan,
Niu JianMing
Publication year - 2017
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1600157
Subject(s) - biology , loss of heterozygosity , locus (genetics) , microsatellite , genetic diversity , allele , poaceae , genetics , stipa , population , botany , gene , demography , sociology
Premise of the study: Stipa breviflora (Poaceae) is one of the dominant species of the desert steppe in the eastern Eurasian grasslands. Simple sequence repeat (SSR) markers were developed for use in genetic diversity studies of this species. Methods and Results: A total of 1954 potentially polymorphic loci were obtained by comparing transcriptome data of eight different S. breviflora individuals. We selected 81 loci to verify polymorphism and 63 loci amplified, of which 21 loci exhibited polymorphism. The number of alleles per locus varied from two to 24, the observed heterozygosity ranged from 0.083 to 0.958, and the expected heterozygosity ranged from 0.396 to 0.738. Conclusions: These newly identified SSR loci can be used for population genetic and landscape genetic studies of S. breviflora . In addition, 14 loci also amplified in six related Stipa species ( S. grandis, S. krylovii, S. bungeana, S. aliena, S. gobica , and S. purpurea ).