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Premise of the study:   Pseudotaxus chienii  (Taxaceae) is an old rare species endemic to China that has adapted well to ecological heterogeneity with high genetic diversity in its nuclear genome. However, the genetic variation in its chloroplast genome is unknown.    Methods and Results:  Eighteen chloroplast microsatellite markers (cpSSRs) were developed from the whole chloroplast genome of  Taxus chinensis  var.  mairei  and successfully amplified in four  P. chienii  populations and one  T. chinensis  var.  mairei  population. Of these loci, 10 were polymorphic in  P. chienii , whereas six were polymorphic in  T. chinensis  var.  mairei . The unbiased haploid diversity per locus ranged from 0.000 to 0.641 and 0.000 to 0.545 for  P. chienii  and  T. chinensis  var.  mairei , respectively.    Conclusions:  The 18 cpSSRs will be used to further investigate the chloroplast genetic structure and adaptive evolution in  P. chienii  populations.

Chloroplast microsatellite markers for Pseudotaxus chienii developed from the whole chloroplast genome of Taxus chinensis var. mairei (Taxaceae)