Open AccessMicrosatellite primers for the rare shrub Acacia adinophylla (Fabaceae)
Author(s) -
Nevill Paul G.,
WardellJohnson Grant
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1600084
Subject(s) - biology , microsatellite , fabaceae , loss of heterozygosity , genetics , genetic diversity , allele , genetic variation , evolutionary biology , botany , gene , population , demography , sociology
Premise of the study: Microsatellite primers were developed for the rare shrub Acacia adinophylla (Fabaceae) to assess genetic diversity and its spatial structuring. Methods and Results: Shotgun sequencing on an Illumina MiSeq produced 6,372,575 reads. Using the QDD pipeline, we designed 60 primer pairs, which were screened using PCR. Seventeen loci were developed, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. These loci were then screened for variation in individuals from three populations. The number of alleles observed for these 12 loci ranged from three to 18 and expected heterozygosity ranged from 0.13 to 0.85. Conclusions: These markers will enable the quantification of genetic impact of proposed mining activities on the short‐range endemic Acacia adinophylla .