Open AccessPolymorphic microsatellite markers in Anthoxanthum (Poaceae) and cross‐amplification in the Eurasian complex of the genus
Author(s) -
LemaSuárez Irene,
Sahuquillo Elvira,
MaríMeeus,
Pimentel Manuel
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1600070
Subject(s) - biology , polyploid , microsatellite , reticulate evolution , ploidy , botany , genetics , evolutionary biology , phylogenetics , allele , gene
Premise of the study: Nonplastid microsatellite primers were developed for the first time in the Euro‐Siberian complex of Anthoxanthum (Poaceae), a genus of temperate grasses in which reticulate evolution is common. Methods and Results: A microsatellite‐enriched genomic DNA library allowed the detection of 500 fragments containing a microsatellite motif. Fifteen primer pairs were selected for an extended primer test. A preliminary analysis was conducted on the Eurasian diploid lineages of Anthoxanthum , with special emphasis on three populations of the Mediterranean A. aristatum – A. ovatum complex. Thirteen out of 15 markers tested were polymorphic in the complex, with successful cross‐amplification in A. odoratum (93% polymorphic loci), A. amarum (73% polymorphic), A. alpinum (73% polymorphic), and A. maderense (60% polymorphic). Conclusions: These microsatellite markers will enable the analysis of evolution and phylogeography in diploid and polyploid lineages of this important genus.