Open AccessDevelopment and characterization of 27 microsatellite markers for the mangrove fern, Acrostichum aureum (Pteridaceae)
Author(s) -
Yamamoto Takashi,
Tsuda Yoshiaki,
Mori Gustavo Maruyama,
Cruz Mariana Vargas,
Shinmura Yoshimi,
Wee Alison K. S.,
Takayama Koji,
Asakawa Takeshi,
Yamakawa Takeru,
Suleiman Monica,
NúñezFarfán Juan,
Webb Edward L.,
Watano Yasuyuki,
Kajita Tadashi
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1600042
Subject(s) - biology , pteridaceae , microsatellite , fern , pantropical , mangrove , botany , zoology , genetics , ecology , allele , genus , gene
Premise of the study: Twenty‐seven nuclear microsatellite markers were developed for the mangrove fern, Acrostichum aureum (Pteridaceae), to investigate the genetic structure and demographic history of the only pantropical mangrove plant. Methods and Results: Fifty‐six A. aureum individuals from three populations were sampled and genotyped to characterize the 27 loci. The number of alleles and expected heterozygosity ranged from one to 15 and 0.000 to 0.893, respectively. Across the 26 polymorphic loci, the Malaysian population showed much higher levels of polymorphism compared to the other two populations in Guam and Brazil. Cross‐amplification tests in the other two species from the genus determined that seven and six loci were amplifiable in A. danaeifolium and A. speciosum , respectively. Conclusions: The 26 polymorphic microsatellite markers will be useful for future studies investigating the genetic structure and demographic history of of A. aureum , which has the widest distributional range of all mangrove plants.