Open AccessA workflow to preserve genome‐quality tissue samples from plants in botanical gardens and arboreta
Author(s) -
Gostel Morgan R.,
Kelloff Carol,
Wallick Kyle,
Funk Vicki A.
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1600039
Subject(s) - workflow , biology , herbarium , sampling (signal processing) , plan (archaeology) , genome , quality (philosophy) , data science , ecology , database , engineering , computer science , genetics , gene , electrical engineering , philosophy , epistemology , paleontology , filter (signal processing)
Premise of the study: Internationally, gardens hold diverse living collections that can be preserved for genomic research. Workflows have been developed for genomic tissue sampling in other taxa (e.g., vertebrates), but are inadequate for plants. We outline a workflow for tissue sampling intended for two audiences: botanists interested in genomics research and garden staff who plan to voucher living collections. Methods and Results: Standard herbarium methods are used to collect vouchers, label information and images are entered into a publicly accessible database, and leaf tissue is preserved in silica and liquid nitrogen. A five‐step approach for genomic tissue sampling is presented for sampling from living collections according to current best practices. Conclusions: Collecting genome‐quality samples from gardens is an economical and rapid way to make available for scientific research tissue from the diversity of plants on Earth. The Global Genome Initiative will facilitate and lead this endeavor through international partnerships.