Open AccessNew development and validation of 50 SSR markers in breadfruit ( Artocarpus altilis , Moraceae) by next‐generation sequencing
Author(s) -
De Bellis Fabien,
Malapa Roger,
Kagy Valérie,
Lebegin Stéphane,
Billot Claire,
Labouisse JeanPierre
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1600021
Subject(s) - biology , moraceae , microsatellite , genotyping , locus (genetics) , artocarpus , genetic diversity , dna sequencing , allele , botany , genotype , genetics , gene , population , demography , sociology
Premise of the study: Using next‐generation sequencing technology, new microsatellite loci were characterized in Artocarpus altilis (Moraceae) and two congeners to increase the number of available markers for genotyping breadfruit cultivars. Methods and Results: A total of 47,607 simple sequence repeat loci were obtained by sequencing a library of breadfruit genomic DNA with an Illumina MiSeq system. Among them, 50 single‐locus markers were selected and assessed using 41 samples (39 A. altilis , one A. camansi , and one A. heterophyllus ). All loci were polymorphic in A. altilis , 44 in A. camansi , and 21 in A. heterophyllus . The number of alleles per locus ranged from two to 19. Conclusions: The new markers will be useful for assessing the identity and genetic diversity of breadfruit cultivars on a small geographical scale, gaining a better understanding of farmer management practices, and will help to optimize breadfruit genebank management.