Open AccessMicrosatellite primers for a species of South African everlasting daisy ( Helichrysum odoratissimum ; Gnaphalieae, Asteraceae)
Author(s) -
Glen Kelsey L.,
Cron Glynis V.
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500138
Subject(s) - biology , gene flow , microsatellite , ploidy , asteraceae , locus (genetics) , allele , genetics , primer (cosmetics) , evolutionary biology , botany , gene , genetic variation , chemistry , organic chemistry
Premise of the study: Microsatellites were developed for the widespread Helichrysum odoratissimum (Asteraceae) to estimate gene flow across diploid populations and to test if gene flow occurs among other closely related lineages within this genus. Methods and Results: Ten primer pairs were developed and tested using populations across South Africa; however, only seven primer pairs were polymorphic for the target species. The seven polymorphic primers amplified di‐ and trinucleotide repeats with up to 16 alleles per locus among 125 diploid individuals used for analyses. Conclusions: These markers can be used to estimate gene flow among populations of known ploidy level of H. odoratissimum to test evolutionary hypotheses. Furthermore, these markers amplify successfully in other Helichrysum species, including the other three taxonomic Group 4 species, and therefore can be used to inform taxonomic work on these species.