Open AccessDevelopment and characterization of microsatellite primers for Chamaecyparis obtusa (Cupressaceae)
Author(s) -
Kim Young Mi,
Shin Yu Seung,
Jeong Ji Hee
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500136
Subject(s) - chamaecyparis , microsatellite , biology , cupressaceae , genetic marker , evolutionary biology , genetics , botany , gene , pollen , allele
Premise of the study: Although several microsatellite markers of Chamaecyparis obtusa (Cupressaceae) have been reported in previous studies, we newly developed and evaluated polymorphic microsatellite markers of C. obtusa , an economically important species in Korea as a major forestation tree species, for further genetic studies. Methods and Results: Microsatellite‐enrichment libraries were constructed according to the selective hybridization method. From this output, we obtained 10 polymorphic and five monomorphic microsatellite markers for C. obtusa . Five of these developed markers were successfully amplified for C. pisifera , a related species. Conclusions: These microsatellite markers can be valuable tools for further genetic studies of C. obtusa , and gathered genetic information may be useful for breeding program management.