Open AccessCharacterization of microsatellites identified by next‐generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)
Author(s) -
Morillo Eduardo,
Buitron Johanna,
Limongi Ricardo,
Vignes Helene,
Argout Xavier
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500135
Subject(s) - biology , bignoniaceae , microsatellite , genetic diversity , evolutionary biology , ecology , genetics , population , allele , gene , demography , sociology
Premise of the study: We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador. Methods and Results: Using next‐generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii . Conclusions: We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species.