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Premise of the study:  Twenty‐six microsatellite (simple sequence repeat [SSR]) markers were characterized in  Psychotria homalosperma  (Rubiaceae), an endemic evergreen tree in the Bonin Islands, Japan, to investigate the genetic structure and gene flow of the species.    Methods and Results:  Using next‐generation sequencing, we developed 26 SSR markers for  P. homalosperma  with perfect motifs from di‐ to pentanucleotide repeats. Of these, the Chichijima and Hahajima island populations of  P. homalosperma  had mean allele numbers of 6.50 and 6.81, respectively. The mean expected heterozygosities were 0.578 and 0.606, respectively. In addition, 10 and eight of these markers were successfully amplified for  P. boninensis  and  P. serpens , respectively, occurring in the same or adjacent areas.    Conclusions:  The SSR markers developed in this study will be useful for future studies concerning the population genetics of  P. homalosperma  and will facilitate the development of a conservation strategy.

Development of SSR markers for Psychotria homalosperma (Rubiaceae) and cross‐amplification in four other species