Open AccessPolymorphic microsatellite loci for Haumania danckelmaniana and transferability to H. liebrechtsiana (Marantaceae)
Author(s) -
Ley Alexandra C.,
Hardy Olivier J.
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500116
Subject(s) - microsatellite , biology , locus (genetics) , transferability , gene flow , allele , genetic diversity , genetics , evolutionary biology , gene , genetic variation , population , statistics , demography , mathematics , logit , sociology
Premise of the study: Microsatellite markers were developed for the species Haumania danckelmaniana (Marantaceae) from central tropical Africa. Methods and Results: Microsatellite isolation was performed simultaneously on three different species of Marantaceae through a procedure that combines multiplex microsatellite enrichment and next‐generation sequencing. From 80 primers selected for initial screening, 20 markers positively amplified in H. danckelmaniana , of which 10 presented unambiguous amplification products within the expected size range and eight were polymorphic with four to nine alleles per locus. Positive transferability with the related species H. liebrechtsiana was observed for the same 10 markers. Conclusions: The polymorphic microsatellite markers are suitable for studies in genetic diversity and structure, mating system, and gene flow in H. danckelmaniana and the closely related species H. liebrechtsiana .