Open AccessDevelopment and characterization of polymorphic microRNA‐based microsatellite markers in Nelumbo nucifera (Nelumbonaceae)
Author(s) -
Wang Xiaolei,
Gui Songtao,
Pan Lei,
Hu Jihong,
Ding Yi
Publication year - 2016
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500091
Subject(s) - biology , microsatellite , genetic diversity , locus (genetics) , loss of heterozygosity , genetics , allele , primer (cosmetics) , population , evolutionary biology , genetic marker , gene , chemistry , demography , organic chemistry , sociology
Premise of the study: Polymorphic microRNA (miRNA)–based microsatellite markers were developed to investigate the genetic diversity and population structure of Nelumbo nucifera (Nelumbonaceae). Methods and Results: A total of 485 miRNA‐based microsatellites were found from the genomic DNA sequences of N. nucifera . After several rounds of screening, 21 primer pairs flanking di‐, tri‐, or pentanucleotide repeats were identified that revealed high levels of genetic diversity in four populations with two to five alleles per locus. The observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.803, respectively. Conclusions: The polymorphic microsatellite markers will be useful for studying the genetic diversity and population structure of N. nucifera .