Open AccessDevelopment of microsatellite markers for the endangered Pedicularis ishidoyana (Orobanchaceae) using next‐generation sequencing
Author(s) -
Cho WonBum,
Choi InSu,
Choi ByoungHee
Publication year - 2015
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500083
Subject(s) - biology , microsatellite , orobanchaceae , endangered species , threatened species , population , conservation genetics , evolutionary biology , primer (cosmetics) , genetics , allele , botany , ecology , habitat , gene , chemistry , demography , organic chemistry , sociology
Premise of the study: Microsatellite primers were developed for Pedicularis ishidoyana (Orobanchaceae), an endangered and hemiparasitic plant that is narrowly endemic to Korea. Because its populations are threatened by loss of suitable habitat, conservation efforts are required. Methods and Results: We developed polymorphic microsatellite loci through reference mapping of 300‐bp paired‐end reads obtained from Illumina MiSeq data. In all, 74 primer pairs were designed and 32 were amplified. Of these, 18 pairs were polymorphic, with two to six alleles each occurring in 26 individual plants. Expected and observed heterozygosities ranged from 0.142 to 0.703 and from 0.077 to 0.615, respectively. Conclusions: These microsatellite markers are expected to be useful for studies of the population genetics of P. ishidoyana .