Open AccessMicrosatellite markers isolated from Cabomba aquatica s.l. (Cabombaceae) from an enriched genomic library
Author(s) -
Barbosa Tiago D. M.,
Trad Rafaela J.,
Bajay Miklos M.,
Amaral Maria C. E.
Publication year - 2015
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500076
Subject(s) - biology , microsatellite , loss of heterozygosity , locus (genetics) , genetics , genetic diversity , fixation index , population , allele , evolutionary biology , gene , demography , sociology
Premise of the study: Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. Methods and Results: Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loci were polymorphic, and alleles per locus ranged from two to four across the 49 C. aquatica s.l. individuals. Observed heterozygosity, expected heterozygosity, and fixation index varied from 0.0 to 1.0, 0.0 to 0.5, and −1.0 to −0.0667, respectively, for the Manaus population and from 0.0 to 1.0, 0.0 to 0.6, and −1.0 to 0.4643 for the Viruá population. Conclusions: The developed markers will be used in further taxonomic and population studies within Cabomba . This set of microsatellite primers represents the first report on rapid molecular markers in the genus.