Open AccessDevelopment of polymorphic microsatellite markers for the Killarney Fern ( Vandenboschia speciosa , Hymenophyllaceae)
Author(s) -
GarcíaLópez M. del Carmen,
Schuler Samira BenMenni,
LópezFlores Inmaculada,
NietoLugilde Marta,
TerrónCamero Laura,
Aguilera Ismael Mazuecos,
SuárezSantiago Víctor N.
Publication year - 2015
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500067
Subject(s) - biology , microsatellite , sporophyte , genetic diversity , fern , loss of heterozygosity , population , endangered species , evolutionary biology , genetic marker , genetics , allele , botany , ecology , gene , demography , sociology , habitat
Premise of the study: We characterize 10 microsatellite loci in the endangered fern Vandenboschia speciosa (Hymenophyllaceae), enabling studies on the genetic population structure of this Macaronesian‐European species using DNA hypervariable markers. Methods and Results: Ten primer sets were developed and tested on 47 individuals in a total of two Iberian populations of V. speciosa . The primers amplified di‐ and hexanucelotide repeats. The number of alleles ranged from two to eight, and the expected heterozygosity ranged from 0.107 to 0.807 among the populations analyzed. Conclusions: The 10 microsatellite markers developed will be useful in characterizing the genetic diversity of V. speciosa and understanding its population structure (including the possible structure between sporophyte and gametophyte phases) and biogeographic history, and will provide important genetic data for the conservation of this species.