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Premise of the study:  Microsatellite primers were developed in the perennial herbs of the diploid‐polyploid complex  Veronica  subsect.  Pentasepalae  (Plantaginaceae) to investigate the role that hybridization has played in the evolution of the group, which includes several endangered species.    Methods and Results:  Twelve pairs of primers leading to polymorphic and readable markers were identified and optimized from  V. jacquinii  and  V. orbiculata  using a microsatellite‐enriched library method and 454 GS‐FLX technique. The set of primers amplified dinucleotide to pentanucleotide repeats, and the number of alleles per locus ranged from one to six, one to 11, and one to nine for  V. orsiniana, V. javalambrensis , and  V. rosea , respectively. Transferability analyses were performed in 20 species corresponding to 10 different subgenera.    Conclusions:  These results indicate the utility of the newly developed microsatellites across  Veronica  subsect.  Pentasepalae , which will help in the study of gene flow patterns and genetic structure.

Characterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross‐amplification in 10 other subgenera