Open AccessIsolation of nuclear microsatellites in the African timber tree Lophira alata (Ochnaceae) and cross‐amplification in L. lanceolata
Author(s) -
Piñeiro Rosalía,
Staquet Adrien,
Hardy Olivier J.
Publication year - 2015
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500056
Subject(s) - biology , microsatellite , isolation (microbiology) , evolutionary biology , zoology , genetics , bioinformatics , gene , allele
Premise of the study: Microsatellite markers were isolated in the rainforest tree Lophira alata (Ochnaceae), an important timber tree from Central Africa, and cross‐amplified on its savannah counterpart, L. lanceolata . Methods and Results: From a microsatellite‐enriched library sequenced on a 454 GS FLX platform, 13 primer combinations were identified. Amplification was optimized in two multiplex reactions. The primers amplified di‐ and trinucelotide repeats, with two to seven alleles per locus. Eleven primers also amplified in L. lanceolata . Conclusions: Microsatellite primers developed for the genus Lophira displayed sufficient variation to investigate hybridization between congeneric species in the rainforest–savannah transition.