Open AccessDevelopment of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae)
Author(s) -
Sakaguchi Shota,
Lannuzel Guillaume,
Fogliani Bruno,
Wulff Adrien S.,
L'Huillier Laurent,
Kurata Seikan,
Ueno Saneyoshi,
Isagi Yuji,
Tsumura Yoshihiko,
Ito Motomi
Publication year - 2015
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500045
Subject(s) - biology , microsatellite , genetic diversity , cupressaceae , endangered species , evolutionary biology , loss of heterozygosity , chloroplast dna , genetic marker , allele , genetics , botany , population , chloroplast , ecology , gene , pollen , demography , sociology , habitat
Premise of the study: Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia. Methods and Results: Using sequencing by synthesis (SBS) of an RNA‐Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were developed. When evaluated with 48 individuals, these markers showed genetic variations ranging from two to 15 alleles and expected heterozygosity ranging from 0 to 0.881. Conclusions: These markers will be useful for examining the genetic diversity and structure of remaining wild populations and improving the genetic status of ex situ populations.