Open AccessDevelopment of microsatellite markers in Ilex kaushue (Aquifoliaceae), a medicinal plant species
Author(s) -
Qin LanFang,
Qu XinCheng,
Hu Gang,
Huang YunFeng,
Zhang QiWei
Publication year - 2015
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500040
Subject(s) - biology , microsatellite , loss of heterozygosity , genetic diversity , locus (genetics) , allele , pyrosequencing , genetic marker , genetics , botany , evolutionary biology , population , gene , demography , sociology
Premise of the study: Microsatellite markers were developed for Ilex kaushue (Aquifoliaceae), a medicinal plant with extremely small wild populations that exists in fragmented habitats, to assess and protect its genetic diversity. Methods and Results: Using 454 GS FLX Titanium sequencing, 16 microsatellite primer sets were isolated and characterized. Fifteen of these markers were polymorphic. The number of alleles per locus ranged from one to nine across 22 individuals from both cultivated and wild populations. The observed and expected heterozygosity in these two populations ranged from 0.000 to 1.000 and from 0.000 to 0.785, respectively. Conclusions: These markers will be useful in studies on genetic diversity of I. kaushue .