Open AccessIsolation and characterization of 12 microsatellite loci in soapbark, Quillaja saponaria (Quillajaceae)
Author(s) -
Letelier Luis,
Harvey Nick,
Valderrama Aly,
Stoll Alexandra,
GonzálezRodríguez Antonio
Publication year - 2015
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500024
Subject(s) - biology , microsatellite , genetic diversity , locus (genetics) , intraspecific competition , allele , population , genetics , evolutionary biology , zoology , gene , demography , sociology
Premise of the study: Microsatellite primers were developed for the endemic Chilean tree Quillaja saponaria (Quillajaceae), a common member of the sclerophyllous Mediterranean forest, to investigate intraspecific patterns of genetic diversity and structure. Methods and Results: Using an enriched library, 12 polymorphic microsatellite loci were developed in Q. saponaria . All loci consisted of dinucleotide repeats. The average number of alleles per locus was 5.3 (2–13), with a total of 64 alleles recorded in 39 individuals from three populations. Conclusions: The microsatellite markers described here are the first characterized for Q. saponaria . The polymorphic loci will be useful in studies of genetic diversity and genetic population differentiation in natural populations of this species.