Open AccessCharacterization of microsatellite loci for an Australian epiphytic orchid, Dendrobium calamiforme , using Illumina sequencing
Author(s) -
Trapnell Dorset W.,
Beasley Rochelle R.,
Lance Stacey L.,
Field Ashley R.,
Jones Kenneth L.
Publication year - 2015
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500016
Subject(s) - biology , microsatellite , genetic diversity , locus (genetics) , gene flow , loss of heterozygosity , genetics , dendrobium , evolutionary biology , population , allele , genetic variation , botany , gene , demography , sociology
Premise of the study: Microsatellite loci were developed for the epiphytic pencil orchid Dendrobium calamiforme for population genetic and phylogeographic investigation of this Australian taxon. Methods and Results: Nineteen microsatellite loci were identified from an Illumina paired‐end shotgun library of D. calamiforme . Polymorphism and genetic diversity were assessed in 24 individuals from five populations separated by a maximum distance of ∼80 km. All loci were polymorphic with two to 14 alleles per locus, expected heterozygosity ranging from 0.486 to 0.902, and probability of identity values ranging from 0.018 to 0.380. Conclusions: These novel markers will serve as valuable tools for investigation of levels of genetic diversity as well as patterns of gene flow, genetic structure, and phylogeographic history.