Open AccessDevelopment and characterization of microsatellite loci for the endangered scrub lupine, Lupinus aridorum (Fabaceae)
Author(s) -
Ricono Angela,
Bupp Glen,
Peterson Cheryl,
Nunziata Schyler O.,
Lance Stacey L.,
Pruett Christin L.
Publication year - 2015
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1500013
Subject(s) - biology , endangered species , fabaceae , lupinus , microsatellite , genetic diversity , locus (genetics) , botany , allele , ecology , genetics , population , demography , sociology , habitat , gene
Premise of the study: Microsatellite primers were developed in scrub lupine ( Lupinus aridorum , Fabaceae), an endemic species to Florida that is listed as endangered in the United States, to assess connectivity among populations, identify hybrids, and examine genetic diversity. Methods and Results: We isolated and characterized 12 microsatellite loci polymorphic in scrub lupine or in closely related species (i.e., sky‐blue lupine [ L. diffusus ] and Gulf Coast lupine [ L. westianus ]). Loci showed low to moderate polymorphism, ranging from two to 14 alleles per locus and 0.01 to 0.86 observed heterozygosity. Conclusions: These loci are the first developed for Florida species of lupine and will be used to determine differentiation among species and to aid in conservation of the endangered scrub lupine.