Open AccessDevelopment and characterization of chloroplast microsatellite markers in a fine‐leaved fescue, Festuca rubra (Poaceae)
Author(s) -
von Cräutlein Maria,
Korpelainen Helena,
Helander Marjo,
Väre Henry,
Saikkonen Kari
Publication year - 2014
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1400094
Subject(s) - biology , festuca rubra , microsatellite , intergenic region , festuca , population , botany , chloroplast dna , endophyte , poaceae , allele , chloroplast , genome , genetics , gene , demography , sociology
• Premise of the study: Chloroplast microsatellite markers were developed for Festuca rubra to examine its population genetic characteristics, taxonomy, and coevolution with its endophyte Epichloë festucae . • Methods and Results: Thirteen polymorphic markers were identified from the chloroplast genome of a F. ovina accession and intergenic chloroplast sequences of F. rubra accessions. They amplified a total of 65 alleles in a sample of 93 individuals of F. rubra originating from six different populations located in the Faroe Islands, Finland, Greenland, Norway, and Spain. • Conclusions: The developed microsatellite primer pairs can be used by researchers in population genetic and taxonomic studies, and by plant breeders in breeding programs on grasses.