Open AccessMicrosatellite primers for the fungi Rhizopogon kretzerae and R. salebrosus (Rhizopogonaceae) from 454 shotgun pyrosequencing
Author(s) -
Grubisha Lisa C.,
Brewer Jessica D.,
Dowie Nicholas J.,
Miller Steven L.,
Trowbridge Steven M.,
Klooster Matthew R.
Publication year - 2014
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1400029
Subject(s) - biology , microsatellite , pyrosequencing , shotgun sequencing , loss of heterozygosity , population , genetics , allele , evolutionary biology , dna sequencing , gene , sociology , demography
• Premise of the study: Rhizopogon kretzerae and R. salebrosus (Rhizopogonaceae) are ectomycorrhizal fungi symbiotic with pines and the mycoheterotrophic plant Pterospora andromedea (Ericaceae). Microsatellite loci will allow population genetic study of fungal hosts to P. andromedea . • Methods and Results: Shotgun pyrosequencing of R. kretzerae DNA resulted in primer development of 23 perfect microsatellite loci and screened across two populations each for R. kretzerae and R. salebrosus . Twelve loci were polymorphic in R. kretzerae populations, and 11 loci cross‐amplified in R. salebrosus populations. For R. kretzerae and R. salebrosus , number of alleles was one to eight and one to nine, respectively, and observed heterozygosity ranged from 0.00–0.57 and 0.00–0.70, respectively. • Conclusions: These are the first microsatellite loci developed for any species within Rhizopogon subgenus Amylopogon . These microsatellite loci will be used in conservation genetic studies of rare to endangered eastern populations and to compare plant and fungal population genetic structure at different hierarchical levels.