Open AccessDevelopment of microsatellite markers for Vitex rotundifolia (Verbenaceae), an endangered coastal plant in Lake Biwa, Japan
Author(s) -
Ohtsuki Tatsuo,
Shoda Tasuku,
Kaneko Yuko,
Setoguchi Hiroaki
Publication year - 2014
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1300100
Subject(s) - biology , endangered species , genetic diversity , microsatellite , locus (genetics) , population , ecology , zoology , allele , genetics , gene , habitat , demography , sociology
• Premise of the study: Microsatellite loci were developed for Vitex rotundifolia , an endangered species isolated to Lake Biwa, to investigate its genetic diversity and population structure. • Methods and Results: Ten primer sets were identified in Japanese populations of V. rotundifolia . The number of alleles per locus ranged from one to six and gene diversity per locus ranged from 0.040 to 0.697 between two populations. In addition, all loci could be successfully amplified in V. trifolia . • Conclusions: These markers will be useful for studies of genetic diversity and population structure of endangered species isolated to Lake Biwa of V. rotundifolia , to aid in the development of conservation strategies.