Open AccesscpDNA microsatellite markers for Lemna minor (Araceae): Phylogeographic implications
Author(s) -
Wani Gowher A.,
Shah Manzoor A.,
Reshi Zafar A.,
Atangana Alain R.,
Khasa Damase P.
Publication year - 2014
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1300099
Subject(s) - biology , microsatellite , chloroplast dna , haplotype , genetics , genotyping , population , phylogeography , minor allele frequency , evolutionary biology , population genetics , allele , genotype , chloroplast , allele frequency , phylogenetics , gene , demography , sociology
• Premise of the study: A lack of genetic markers impedes our understanding of the population biology of Lemna minor . Thus, the development of appropriate genetic markers for L. minor promises to be highly useful for population genetic studies and for addressing other life history questions regarding the species. • Methods and Results: For the first time, we characterized nine polymorphic and 24 monomorphic chloroplast microsatellite markers in L. minor using DNA samples of 26 individuals sampled from five populations in Kashmir and of 17 individuals from three populations in Quebec. Initially, we designed 33 primer pairs, which were tested on genomic DNA from natural populations. Nine loci provided markers with two alleles. Based on genotyping of the chloroplast DNA fragments from 43 sampled individuals, we identified one haplotype in Quebec and 11 haplotypes in Kashmir, of which one occurs in 56% of the genotypes, one in 8%, and nine in 4%, respectively. There was a maximum of two alleles per locus. • Conclusions: These new chloroplast microsatellite markers for L. minor and haplotype distribution patterns indicate a complex phylogeographic history that merits further investigation.