Open AccessDevelopment and characterization of 10 microsatellite markers in Sagina nodosa (Caryophyllaceae)
Author(s) -
Singh Tarjinder,
Edwards Joan,
Maroja Luana S.
Publication year - 2014
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1300064
Subject(s) - microsatellite , biology , locus (genetics) , genetics , contig , evolutionary biology , allele , genome , gene
• Premise of the study: We developed 10 novel microsatellite loci for Sagina nodosa , a diploid perennial arctic‐alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. • Methods and Results: We performed a low‐coverage 454 next‐generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs containing potential polymorphic microsatellites. We present data for 10 novel polymorphic microsatellite loci containing di‐, tri‐, tetra‐, and hexanucleotide repeats with two to nine alleles per locus assessed in 29 individuals. • Conclusions: These polymorphic microsatellite loci in S. nodosa will provide insights on the population structure and life history of S. nodosa in Isle Royale and other North American populations.