Open AccessNew microsatellite markers for wild and commercial species of Passiflora (Passifloraceae) and cross‐amplification
Author(s) -
CerqueiraSilva Carlos B. M.,
Santos Elisa S. L.,
Vieira João G. P.,
Mori Gustavo M.,
Jesus Onildo N.,
Corrêa Ronan X.,
Souza Anete P.
Publication year - 2014
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1300061
Subject(s) - passiflora , biology , microsatellite , passifloraceae , genetic diversity , locus (genetics) , loss of heterozygosity , allele , population , passion fruit , genetics , evolutionary biology , botany , gene , horticulture , demography , sociology
• Premise of the study: We developed the first microsatellites for Passiflora setacea and characterized new sets of markers for P. edulis and P. cincinnata , enabling further genetic diversity studies to support the conservation and breeding of passion fruit species. • Methods and Results: We developed 69 microsatellite markers and, in conjunction with assessments of cross‐amplification using primers available from the literature, present 43 new polymorphic microsatellite loci for three species of Passiflora . The mean number of alleles per locus was 3.1, and the mean values of the expected and observed levels of heterozygosity were 0.406 and 0.322, respectively. • Conclusions: These microsatellite markers will be valuable tools for investigating the genetic diversity and population structure of wild and commercial species of passion fruit ( Passiflora spp.) and may be useful for developing conservation and improvement strategies by contributing to the understanding of the mating system and hybridization within the genus.