Open AccessDevelopment and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae)
Author(s) -
Ison Jennifer L.,
Wagenius Stuart,
Reitz Diedre,
Ashley Mary V.
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1300049
Subject(s) - biology , microsatellite , echinacea (animal) , gene flow , genetic structure , population , genetic diversity , population genetics , perennial plant , allele , genetics , evolutionary biology , genetic variation , botany , ecology , gene , demography , sociology
• Premise of the study: Microsatellite loci for the native prairie perennial Echinacea angustifolia were developed and evaluated for future use in population structure and paternity studies. • Methods and Results: A total of 50 trinucleotide microsatellite regions were identified though an enrichment protocol that prescreens for microsatellite repeats before ligating into a vector. Of these, 11 loci were polymorphic and in Hardy–Weinberg equilibrium in three populations with varying numbers of plants. The loci had between three and 14 alleles and collectively provided high paternity exclusion probabilities. • Conclusions: These sets of microsatellite primers will provide researchers and land managers with valuable information on the population genetic structure and gene flow between fragmented prairie populations.