Open AccessIsolation, via 454 sequencing, and characterization of microsatellites for Vachellia farnesiana (Fabaceae: Mimosoideae)
Author(s) -
Bell Karen L.,
Murphy Daniel J.,
Gardner Michael G.
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1300035
Subject(s) - biology , microsatellite , locus (genetics) , loss of heterozygosity , genetics , shotgun sequencing , allele , evolutionary biology , genetic variation , dna sequencing , gene
• Premise of the study: We isolated 15 polymorphic microsatellite markers from Vachellia farnesiana for use in population genetic studies to determine the native range of the species. • Methods and Results: Initially, 454 shotgun sequencing was used to identify and design primers for 68 microsatellite loci. Of these, we trialed 47 loci in the target species, and 42 (89%) amplified a product of expected size. Fifteen of the 47 loci were screened for variation in 21 individuals from the native range of V. farnesiana in southern Mexico and 20 from northwestern Australia. Fourteen loci were polymorphic, with observed heterozygosity ranging from 0.026 to 1.00 (mean = 0.515) and two to 12 alleles per locus (average = 5.2). Cross‐amplification was successful in four to 11 loci in three other Vachellia species. • Conclusions: The new microsatellite loci will be useful in understanding genetic variation and investigating the role of human‐mediated dispersal in the current distribution of V. farnesiana .