Open AccessIsolation and characterization of novel EST‐derived genic markers in Pisum sativum (Fabaceae)
Author(s) -
Jain Shalu,
McPhee Kevin E.
Publication year - 2013
Publication title -
applications in plant sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 23
ISSN - 2168-0450
DOI - 10.3732/apps.1300026
Subject(s) - biology , pisum , sativum , medicago truncatula , fabaceae , expressed sequence tag , genetic marker , botany , genetics , homology (biology) , gene , genetic diversity , transferability , complementary dna , symbiosis , bacteria , population , statistics , demography , mathematics , logit , sociology
• Premise of the study: Novel markers were developed for pea ( Pisum sativum ) from pea expressed sequence tags (ESTs) having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross‐species transferability. • Methods and Results: Seventy‐seven EST‐derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. • Conclusions: The novel markers described here will be useful for future genetic studies of P. sativum ; their amplification in lentil ( Lens culinaris ) demonstrates their potential for use in closely related species.